| Autor: |
WANG Hai-yan, GAO Wen-ting, HE Qing-hua, YANG Ce, GU Wei, YAN Jun, JIANG Jian-xin |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
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| Zdroj: |
Chinese Journal of Traumatology, Vol 15, Iss 6, Pp 323-328 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
1008-1275 |
| Popis: |
【Abstract】 Objective: To explore the changes of Treg-Th17 balance influenced by corticosterone, major ef-fect hormone of hypothalamic-pituitary-adrenal (HPA) axis under running stress. Methods: A total of 25 corticotropin-releasing hor-mone (CRH) wildtype (CRH+/+) and knockout (CRH-/-) mice were adopt and divided into 4 groups as follows: CRH+/+ ctrl, CRH+/+ stress, CRH-/- ctrl and CRH-/- stress. All mice in stress groups were under 2 h running. After 1 h, blood plasma in all groups was collected and the ex-pression of corticosterone and IL-17A was detected by ELISA. Meanwhile, unicell suspensions of peripheral lymph node and spleen in each group were prepared too and stained by PE-CD4 and FITC-CD25, then the changes of Treg (CD4+CD25+) in different groups were checked by flow cytometry; all data were statistically analyzed by the software of WinMDI 2.9, SPSS 11.5, Origin 7.5 and Matlab 2-D and 3-D plot function. Results: The levels of corticosterone were signifi-cantly higher in stress groups than that in correspond-ing control groups (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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