Application of an industrial waste magnetic iron dust as a solid phase support for immobilizing enzyme of industrial applications
| Autor: | Jagdish Shantilal Patel, Darshan H Patel, Rucha Desai, Sunil Shah, Piyush Chudasama, Sachin Joshi, Bhargav Patel |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Adsorption Science & Technology, Vol 34 (2016) |
| Druh dokumentu: | article |
| ISSN: | 0263-6174 2048-4038 02636174 |
| DOI: | 10.1177/0263617416661563 |
| Popis: | Magnetic iron dust, a byproduct by many chemical industries that performs the reduction of nitro compounds to amine, was used for laccase immobilization. The characterization of magnetic iron dust was done by X-ray diffraction, Fourier-transform infrared, and dynamic light scattering. Biodegradable polymer, chitosan, was coated on to the magnetic iron dust by reverse phase suspension method, which was confirmed by Fourier-transform infrared analysis. Immobilization of the laccase enzyme was done onto the chitosan-coated and non-coated magnetic iron dust. The immobilization was monitored by Fourier-transform infrared analysis. Binding efficiency, optimum pH, and optimum temperature for these immobilized laccases were investigated. X-ray diffraction pattern of magnetic iron dust confirmed presence of magnetite (Fe 3 O 4 ) and maghemite (γ-Fe 2 O 3 ) with a particle size of 529.6 nm measured by dynamic light scattering. Laccase was immobilized on chitosan-coated and non-coated magnetic iron dust, monitored by Fourier-transform infrared spectra. Binding efficiency of the laccases was found to be 100% onto the coated and non-coated magnetic iron dust and their activity remained to be 63% and 82%, respectively, even after the 10th cycle of their use. The present results demonstrated the applicability of these immobilized laccase system in the industry in terms of their reusability and waste recycling. |
| Databáze: | Directory of Open Access Journals |
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