SNHG15 aids SARS-CoV-2 entry via RABL2A
| Autor: | Samuel Pushparaj, Chaitanya Gandikota, Kishore Vaddadi, Yurong Liang, Lin Liu |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | RNA Biology, Vol 20, Iss 1, Pp 539-547 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1547-6286 1555-8584 15476286 |
| DOI: | 10.1080/15476286.2023.2241755 |
| Popis: | Angiotensin-converting enzyme 2 (ACE2) and several proteins have been identified as entry factors for severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). However, whether long noncoding RNAs are involved in SARS-CoV-2 entry remains unknown. In this study, we investigated the role of small nucleolar RNA host gene 15 (SNHG15) in SARS-CoV-2 entry using a SARS-CoV-2 spike pseudotyped lentivirus with a luciferase reporter. Overexpression of SNHG15 promoted but SNHG15 knockdown limited SARS-CoV-2 entry in a dose- and time-dependent manner. SNHG15 interacted with Rab-like protein 2A (RABL2A). Overexpression and knockdown of RABL2A produced similar effects on SARS-CoV-2 entry as those of SNHG15. Furthermore, RABL2A knockdown abolished the SNHG15-mediated increase in SARS-CoV-2 entry. In conclusion, SNHG15 is a critical regulatory factor that aids SARS-CoV-2 entry through RABL2A. |
| Databáze: | Directory of Open Access Journals |
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