Development of an ic-ELISA and immunochromatographic strip for detection of sparfloxacin in honey

Autor: Jingjing Li, Aimin Zhi, Guochao Jia, Hui Nie, Lianzhong Ai
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Food and Agricultural Immunology, Vol 29, Iss 1, Pp 147-158 (2018)
Druh dokumentu: article
ISSN: 0954-0105
1465-3443
09540105
DOI: 10.1080/09540105.2017.1364706
Popis: In this study, a sensitive monoclonal antibody (mAb) 3D5 against sparfloxacin (SPFX) was generated. Based on the mAb 3D5, an indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) method was developed for the detection of SPFX in honey. The half maximal inhibitory concentration and limit of detection for the ic-ELISA method were 0.12 and 0.02 ng/mL, respectively. The experiments showed a negligible cross-reaction with other drugs. The average recovery rates for spiked SPFX honey extracts ranged from 90% to 101%, indicating an accepted accuracy. Furthermore, a lateral-flow immunochromatographic assay strip method with a cut-off value of 2 ng/mL was developed. Therefore, both of the developed methods are suitable for future use as rapid screening methods to detect and control the content of SPFX residues in honey samples.
Databáze: Directory of Open Access Journals
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