| Autor: |
Hans Tornqvist, Lennart Krabisch, Per Belfrage |
| Jazyk: |
angličtina |
| Rok vydání: |
1974 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 15, Iss 3, Pp 291-294 (1974) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)36810-3 |
| Popis: |
A simple, sensitive, and specific assay for monoacylglycerol hydrolase activity of rat adipose tissue is described. Monoacyl[3H] glycerols with different chain lengths (8–18 carbon atoms) and different degrees of unsaturation in mixed micellar solution with different detergents can be used as substrates. The [3H]glycerol that is produced is isolated in a one-step liquid–liquid partition procedure. For routine purposes monooleoyl[3H]glycerol was found to be the most suitable substrate. A simple method for the chemical synthesis and purification of this substrate in high yield is given. The assay allows rapid serial sampling of enzymatic activity with a high reproducibility. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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