Combi-seq for multiplexed transcriptome-based profiling of drug combinations using deterministic barcoding in single-cell droplets

Autor: L. Mathur, B. Szalai, N. H. Du, R. Utharala, M. Ballinger, J. J. M. Landry, M. Ryckelynck, V. Benes, J. Saez-Rodriguez, C. A. Merten
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Nature Communications, Vol 13, Iss 1, Pp 1-15 (2022)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/s41467-022-32197-0
Popis: Current screens to assess tumour drug resistance require a large amount of material, normally not available from patients. Here the authors report CombiSeq, a scalable microfluidic workflow to screen hundreds of drug combinations in picoliter-size droplets using transcriptome changes as a readout.
Databáze: Directory of Open Access Journals