Evaluation of Interferon-gamma Application for Recognition of Patients Afflicted by Non-healing Cutaneous Leishmaniasis
| Autor: | Mohammad Moafi, Hossein Rezvan, Roya Sherkat, Sayyed Hamid Zarkesh-Esfahani, Roya Taleban, Ali Asilian, Mohammad Ali Nilforoushzadeh, Fariba Jaffary, Marjan Mansourian, Fatemeh Sokhanvari, Nazli Ansari |
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| Jazyk: | English<br />Persian |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Biological Journal of Microorganism, Vol 6, Iss 22, Pp 101-112 (2017) |
| Druh dokumentu: | article |
| ISSN: | 2322-5173 2322-5181 |
| Popis: | Introduction:Different studies undertaken in the animal modeling show that Interferon-gamma deficiency impairs healing process of Leishmania infection. It seems that the level of Interferon-gamma production could also affect the healing duration of Leishmania lesion in humans. The current study aims to investigate the possibility of Interferon-gamma application for recognition of cases afflicted by non-healing Leishmaniasis. Materials and methods: Peripheral blood mononuclear cells (PBMCs) of 32 patients, who were afflicted by healing or non-healing Leishmaniasis, were isolated and the levels of interferon-gamma were determined, using ELISA method. Afterwards, the cut-off point of interferon-gamma to identify patients afflicted by non-healing Leishmaniasis was calculated through ROC-Curve analysis. Furthermore, Leishmanin Skin Test (LST) was performed for every patient. Results: Levels of Interferon-gamma produced by PBMCs stimulated with Soluble Leishmania Antigen (SLA) or Phytohemaglotinine were significantly higher in healing patients, compared with non-healing individuals (p |
| Databáze: | Directory of Open Access Journals |
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