| Autor: |
Lennert Impens, Thomas B. Jacobs, Hilde Nelissen, Dirk Inzé, Laurens Pauwels |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Frontiers in Genome Editing, Vol 4 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2673-3439 |
| DOI: |
10.3389/fgeed.2022.825042 |
| Popis: |
CRISPR/Cas9 genome editing has been used extensively in a wide variety of plant species. Creation of loss-of-function alleles, promoter variants and mutant collections are a few of the many uses of genome editing. In a typical workflow for sexually reproducing species, plants are generated that contain an integrated CRISPR/Cas9 transgene. After editing of the gene of interest, T-DNA null segregants can be identified in the next generation that contain only the desired edit. However, maintained presence of the CRISPR/Cas9 transgene and continued editing in the subsequent generations offer a range of applications for model plants and crops. In this review, we define transgenerational gene editing (TGE) as the continued editing of CRISPR/Cas9 after a genetic cross. We discuss the concept of TGE, summarize the current main applications, and highlight special cases to illustrate the importance of TGE for plant genome editing research and breeding. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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