Purification and characterization of thermostable chitinase from a novel S. maltophilia strain
| Autor: | Javed, S., Hamid, R., Abdin, Z., Ahmad, M. M., Ahmad, M. |
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| Jazyk: | angličtina |
| Rok vydání: | 2013 |
| Předmět: | |
| Zdroj: | Malaysian Journal of Microbiology, Vol 9, Iss 1, Pp 7-12 (2013) |
| Druh dokumentu: | article |
| ISSN: | 1823-8262 2231-7538 |
| Popis: | Aims: The presents study examines the purification and characterization of a chitinase from S. maltophilia SJ602 strainisolated from a soil sample collected from Jamia Hamdard, New Delhi.Methodology and Results: The purification steps included chitin affinity using colloidal chitin as the affinity matrix andcolumn chromatography using Sephadex G-100. The chitinase was purified to 66 fold having a yield of 17%. The molecular weight of the chitinase was found to be around 29 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The pH and temperature optima of the purified chitinase were found to be at pH 5.5 and60 °C, respectively. Conclusion, Significance and Impact of the study: Besides showing a significant yield, the enzyme has a highthermal stability which has its applicability in the recycling of chitin waste. |
| Databáze: | Directory of Open Access Journals |
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