Addition of Wollastonite Fibers to Calcium Phosphate Cement Increases Cell Viability and Stimulates Differentiation of Osteoblast-Like Cells

Autor: Juliana Almeida Domingues, Mariana Motisuke, Celso Aparecido Bertran, Moema A. Hausen, Eliana Aparecida de Rezende Duek, José Angelo Camilli
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: The Scientific World Journal, Vol 2017 (2017)
Druh dokumentu: article
ISSN: 2356-6140
1537-744X
34876758
DOI: 10.1155/2017/5260106
Popis: Calcium phosphate cement (CPC) that is based on α-tricalcium phosphate (α-TCP) is considered desirable for bone tissue engineering because of its relatively rapid degradation properties. However, such cement is relatively weak, restricting its use to areas of low mechanical stress. Wollastonite fibers (WF) have been used to improve the mechanical strength of biomaterials. However, the biological properties of WF remain poorly understood. Here, we tested the response of osteoblast-like cells to being cultured on CPC reinforced with 5% of WF (CPC-WF). We found that both types of cement studied achieved an ion balance for calcium and phosphate after 3 days of immersion in culture medium and this allowed subsequent long-term cell culture. CPC-WF increased cell viability and stimulated cell differentiation, compared to nonreinforced CPC. We hypothesize that late silicon release by CPC-WF induces increased cell proliferation and differentiation. Based on our findings, we propose that CPC-WF is a promising material for bone tissue engineering applications.
Databáze: Directory of Open Access Journals
Externí odkaz: