A method to simultaneously construct up to 12 differently sized Illumina Nextera long mate pair libraries with reduced DNA input, time, and cost
| Autor: | Darren Heavens, Gonzalo Garcia Accinelli, Bernardo Clavijo, Matthew Derek Clark |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 59, Iss 1, Pp 42-45 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/000114310 |
| Popis: | Long mate pair (LMP) or “jump” libraries are invaluable for producing contiguous genome assemblies and assessing structural variation. However the consistent production of high quality (low duplication rate, accurately sized) LMP libraries has proven problematic in many genome projects. Input DNA length and quantity are key issues that can affect success. Here we demonstrate how 12 libraries covering a wide range of jump sizes can be constructed from |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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