Efficient two-frequency ultrasound extraction of β-carotene from the fungus Blakeslea trispora

Autor: Avdeenko Anatoly P., Belova Elena А., Dašić Predrag V., Konovalova Svetlana A., Baklanova Larisa V., Krstić Sanja S., Milosavljević Milutin M.
Jazyk: English<br />Serbian
Rok vydání: 2017
Předmět:
Zdroj: Hemijska Industrija, Vol 71, Iss 4, Pp 329-336 (2017)
Druh dokumentu: article
ISSN: 0367-598X
2217-7426
DOI: 10.2298/HEMIND151110043A
Popis: Carotenoids are known for their lower environmental pollution, lower costs, shorter extraction time compared to regular extraction methods and higher extraction efficiency. Ultrasound has significant advantages in the extraction of a great number of biologically active compounds including carotenoids. Benefits of using ultrasound for extraction of various compounds are numerous and include effective mixing and micro-mixing, more efficient energy transfer, reduced thermal and concentration gradients, reduced temperature. The aim of this work was to investigate the influence of two-frequency ultrasound main parameters such as ultrasonic intensity, sonication time, and temperature. Application of two-frequency ultrasound (simultaneous influence of ultrasound with frequency of 20–46 kHz, intensity of 1.5–2.5 W/cm2 and frequency of 1.0–2.0 MHz, intensity of 2.0–3.0 W/cm2) raises the percentage of β-carotene extraction from biomass of the fungus Blakeslea trispora from 90–92% up to 98–99% in comparison with one frequency ultrasound. The recommended oil temperature for the extraction process is 35°C, because lower temperature leads to reduction of percentage of β-carotene extraction, and higher temperature promotes unjustified increase of expenditure of energy.
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