Characterizing the bio-functionalization of gold surface with total internal reflection fluorescence (TIRF) microscopy
| Autor: | Robin Ehrminger, Kairi Kivirand, Sergei Kopanchuk, Mart Min, Ago Rinken, Toonika Rinken, Tavo Romann |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | Proceedings of the Estonian Academy of Sciences, Vol 69, Iss 1, Pp 27-34 (2020) |
| Druh dokumentu: | article |
| ISSN: | 1736-6046 1736-7530 |
| DOI: | 10.3176/proc.2020.1.02 |
| Popis: | Quality of bioactive surface is crucial for achieving the required sensitivity and selectivity of biosensing systems. Numerous methods are available for the characterization of metal-coated surfaces, but only a few to test the efficacy of biomaterial immobilization and the level of non-specific binding. Herewith we propose to use total internal reflection fluorescence (TIRF) microscopy for the characterization of the surface analyte recognition capacity. Biomolecules were bound onto titanium/gold covered glass using three different self-assembled monolayers (SAM). The surfaces with attached antibodies were evaluated using the specific binding of fluorophore-labeled secondary antibodies and visualized with TIRF. Among studied SAMs, aminothiol layers with glutaraldehyde coupling demonstrated high binding capacity along with excellent homogeneity indicating their suitability for applications in biosensors. |
| Databáze: | Directory of Open Access Journals |
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