A Protein Complex Map of Trypanosoma brucei.
| Autor: | Vahid H Gazestani, Najmeh Nikpour, Vaibhav Mehta, Hamed S Najafabadi, Houtan Moshiri, Armando Jardim, Reza Salavati |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | PLoS Neglected Tropical Diseases, Vol 10, Iss 3, p e0004533 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1935-2727 1935-2735 07661428 |
| DOI: | 10.1371/journal.pntd.0004533 |
| Popis: | The functions of the majority of trypanosomatid-specific proteins are unknown, hindering our understanding of the biology and pathogenesis of Trypanosomatida. While protein-protein interactions are highly informative about protein function, a global map of protein interactions and complexes is still lacking for these important human parasites. Here, benefiting from in-depth biochemical fractionation, we systematically interrogated the co-complex interactions of more than 3354 protein groups in procyclic life stage of Trypanosoma brucei, the protozoan parasite responsible for human African trypanosomiasis. Using a rigorous methodology, our analysis led to identification of 128 high-confidence complexes encompassing 716 protein groups, including 635 protein groups that lacked experimental annotation. These complexes correlate well with known pathways as well as for proteins co-expressed across the T. brucei life cycle, and provide potential functions for a large number of previously uncharacterized proteins. We validated the functions of several novel proteins associated with the RNA-editing machinery, identifying a candidate potentially involved in the mitochondrial post-transcriptional regulation of T. brucei. Our data provide an unprecedented view of the protein complex map of T. brucei, and serve as a reliable resource for further characterization of trypanosomatid proteins. The presented results in this study are available at: www.TrypsNetDB.org. |
| Databáze: | Directory of Open Access Journals |
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