Cellular uptake and cell-to-cell transfer of polyelectrolyte microcapsules within a triple co-culture system representing parts of the respiratory tract
| Autor: | Dagmar A Kuhn, Raimo Hartmann, Kleanthis Fytianos, Alke Petri-Fink, Barbara Rothen-Rutishauser, Wolfgang J Parak |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Science and Technology of Advanced Materials, Vol 16, Iss 3 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1468-6996 1878-5514 |
| DOI: | 10.1088/1468-6996/16/3/034608 |
| Popis: | Polyelectrolyte multilayer microcapsules around 3.4 micrometers in diameter were added to epithelial cells, monocyte-derived macrophages, and dendritic cells in vitro and their uptake kinetics were quantified. All three cell types were combined in a triple co-culture model, mimicking the human epithelial alveolar barrier. Hereby, macrophages were separated in a three-dimensional model from dendritic cells by a monolayer of epithelial cells. While passing of small nanoparticles has been demonstrated from macrophages to dendritic cells across the epithelial barrier in previous studies, for the micrometer-sized capsules, this process could not be observed in a significant amount. Thus, this barrier is a limiting factor for cell-to-cell transfer of micrometer-sized particles. |
| Databáze: | Directory of Open Access Journals |
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