Effect of the complex of probiotic microorganisms of the genus Bacillus on the growth, histological structure of intestines and the resistance of sturgeon hybrid juveniles
| Autor: | Yu. Zabytivskyi, Ya. Tuchapskyi, O. Dobryanska, I. Boretska, O. Vishchur |
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| Jazyk: | English<br />Ukrainian |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Ribogospodarsʹka Nauka Ukraïni, Vol 69, Iss 3, Pp 130-146 (2024) |
| Druh dokumentu: | article |
| ISSN: | 2075-1508 2312-9581 |
| DOI: | 10.61976/fsu2024.03.130 |
| Popis: | Purpose. The purpose of this work is to evaluate the effect of a complex of probiotic microorganisms from the genus Bacillus, namely Bacillus subtilis, B. pumilus, B. licheniformis and B. amyloliquefaciens, as part of the Sviteco-“PWS” preparation, on the growth potential, survival rate, intestinal histostructure and non-specific resistance of age-0+ hybrid sturgeon. Methodology. The object of the study was hybrid juveniles obtained from ♀ bester (♀ Huso huso × ♂ Acipenser ruthenus) and ♂ sterlet (Acipenser ruthenus) (BSS), which were reared in recirculation aquaculture system (RAS) tanks at the Lviv Research Station of the Institute of Fisheries of the National Academy of Agrarian Sciences. The initial weight of the juveniles was 30.69±1.12 g, the stocking density was 30 ind./m2.The duration of the experiment was 28 days. The preparation of the working solution of the “PWC” probiotic complex was carried out by diluting 0.7 dm3 of the preparation concentrate in 50 dm3 of water at a temperature of 35°C. Before feeding, the solution was applied by spraying in an even layer on the feed for the experimental group of fish at the rate of 50 dm3/kg of feed. During cultivation, such fish productive parameters as growth rate, feed conversion ratio and survival rate were periodically studied. At the end of the study, the lysozyme activity of the blood serum was also determined by the nephelometric method, the composition of the intestinal microbiota — by the deep inoculation method, and the histostructure of the medial part of the intestine was studied using light microscopy. The obtained data were processed by variational statistics using MS Excel and Statistica 12.0. The probability of a difference in the results was determined using a one-way analysis of variance (ANOVA) with Tukey’s test. Findings. The positive effect of the probiotic complex was observed in the first 7 days. Next, the fish body used nutrients with approximately the same efficiency in both experimental and control groups and developed linearly. At the end of the experiment, the average weight of sturgeons in the experimental group was 9.57% higher than in the control group (p |
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