Aberrant Expression of miRNA and mRNAs in Lesioned Tissues of Graves' Disease
| Autor: | Qiu Qin, Xuan Wang, Ni Yan, Rong-Hua Song, Tian-Tian Cai, Wen Zhang, Li-Juan Guan, Fatuma-Said Muhali, Jin-An Zhang |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Cellular Physiology and Biochemistry, Vol 35, Iss 5, Pp 1934-1942 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1015-8987 1421-9778 00037400 |
| DOI: | 10.1159/000374002 |
| Popis: | Background and Aims: Abnormal microRNA (miRNA) expression is found in many diseases including autoimmune diseases. However, little is known about the role of miRNA regulation in Graves' disease (GD). Here, we simultaneously detected different expressions of miRNA and mRNAs in thyroid tissues via a high-throughput transcriptomics approach, known as microarray, in order to reveal the relationship between aberrant expression of miRNAs and mRNAs spectrum and GD. Methods: Totally 7 specimens of thyroid tissue from 4 GD patients and 3 controls were obtained by surgery for microarray analysis. Then, 30 thyroid specimens (18 GD and 12 controls) were also collected for further validation by quantitative real-time PCR ( qRT-PCR ). Results: Statistical analysis showed that the expressions of 5 specific miRNA were increased significantly while those of other 18 miRNA were decreased in thyroid tissue of GD patients (FC≥1.3 or≤0.77 and pConclusion: Our study highlights the possibility that miRNA-target gene network may be involved in the pathogenesis of GD and could provide new insights into understanding the pathophysiological mechanisms of GD. |
| Databáze: | Directory of Open Access Journals |
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