Agave syrup as a substrate for inulinase production by Kluyveromyces marxianus NRRL Y-7571
| Autor: | Luana Paula de Azevedo de Oliveira, Tiago Felipe Oliveira, Jonas Contiero, Márcia Luciana Cazetta |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Acta Scientiarum: Biological Sciences, Vol 38, Iss 3 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1679-9283 1807-863X |
| DOI: | 10.4025/actascibiolsci.v38i3.31489 |
| Popis: | The factorial planning was used to plan and optimize inulinase production by the yeast Kluyveromyces marxianus NRRL Y-7571. The experiments were conducted using a Central Composite Design (CCD) 22, at different concentrations of agave syrup (3.6 to 6.4%) and yeast extract (2.2 to 3.0%). After 96 hours of fermentation, the best condition for the inulinase production was 5% agave syrup and 2.5% yeast extract, which yielded an average of 129.21 U mL-1 of inulinase. Partial characterization of the crude enzyme showed that the optimal pH and temperature were 4.0 and 60°C, respectively. The enzyme showed thermal stability at 55°C for 4 hours. |
| Databáze: | Directory of Open Access Journals |
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