Tract ablation after radiofrequency ablation to prevent viable tumor cell adhesion to the needle electrode
| Autor: | Su Jung Ham, Yoonseok Choi, Kyung Won Kim, Young Chul Cho, Dong-Cheol Woo, Choong Wook Lee, Yun-Jin Jang, Pyo Nyun Kim |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | International Journal of Hyperthermia, Vol 37, Iss 1, Pp 1287-1292 (2020) |
| Druh dokumentu: | article |
| ISSN: | 0265-6736 1464-5157 02656736 |
| DOI: | 10.1080/02656736.2020.1846794 |
| Popis: | Purpose To evaluate whether the additive needle tract ablation (TA) can reduce adherent cells on the needle tract after radiofrequency ablation (RFA) in a preclinical HCC mouse model. Methods Hep3B-Luc cells were engrafted in the Balb/c-nude mice. Nineteen mice were randomly assigned into three groups: the needle only group (needle placement only without performing RFA), the RFA only group (needle placement with active RFA treatment), and the RFA-TA group (needle placement with active RFA treatment and additive tract ablation). The 17-gauge needle with a 10-mm active tip was used. After RFA and TA, the viability of adherent tumor cells on the RFA needle was evaluated with bioluminescence imaging (BLI) and live-cell counting. Results We observed that RFA-TA group had the lowest BLI values compared with other groups (needle only group, 11.2 ± 6.4 million; RFA only group, 13.6 ± 9.1 million; RFA-TA group, 1.11 ± 0.8 million, p = 0.001). Live cell counting with acridine orange/propidium iodide staining also confirmed that the counted viable cell numbers in RFA-TA group were lowest compared to the other groups (needle only group, 14.8 ± 4.5; RFA only group, 643.8 ± 131.9; RFA-TA group, 1.5 ± 0.9, p |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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