Photocage-initiated time-resolved solution X-ray scattering investigation of protein dimerization
| Autor: | Inokentijs Josts, Stephan Niebling, Yunyun Gao, Matteo Levantino, Henning Tidow, Diana Monteiro |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | IUCrJ, Vol 5, Iss 6, Pp 667-672 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2052-2525 20522525 |
| DOI: | 10.1107/S2052252518012149 |
| Popis: | This work demonstrates a new method for investigating time-resolved structural changes in protein conformation and oligomerization via photocage-initiated time-resolved X-ray solution scattering by observing the ATP-driven dimerization of the MsbA nucleotide-binding domain. Photocaged small molecules allow the observation of single-turnover reactions of non-naturally photoactivatable proteins. The kinetics of the reaction can be derived from changes in X-ray scattering associated with ATP-binding and subsequent dimerization. This method can be expanded to any small-molecule-driven protein reaction with conformational changes traceable by X-ray scattering where the small molecule can be photocaged. |
| Databáze: | Directory of Open Access Journals |
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