| Autor: |
Esmaeil Mahmoudi, Mahdieh Abolfathi, Naiemeh Hassanzadeh, Yaser Eshaghi Milasi, Mina Dehghani-Samani, Mansoor Khaledi, Hossein Kerdarian, Mina Najafipour, Asghar Arshi |
| Jazyk: |
angličtina |
| Rok vydání: |
2019 |
| Předmět: |
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| Zdroj: |
Translational Medicine Communications, Vol 4, Iss 1, Pp 1-6 (2019) |
| Druh dokumentu: |
article |
| ISSN: |
2396-832X |
| DOI: |
10.1186/s41231-019-0036-5 |
| Popis: |
Abstract Background Apoptosis inhibition depends on the balance between expression of some regulatory genes including Bax and c-FLIP, despite breast cancer has long been associated with disturbances in apoptosis regulation. The objective of present study was to determine the expression levels of Bax and c-FLIP mRNAs in 1 μg sample of total RNA obtained from MCF7 and MDA-MB-231 human breast cancer cells treated with Prunus armeniaca extract. Methods Prunus armeniaca effect was measured by MTT assay with different drug concentrations (0.5, 1, 1.5, 2, 2.5, 3, 3.5, 4, 4.5 and 5 mg/mL) at different incubation times (24, 48 and 72 h). Expression of Bax and c-FLIP mRNA levels was determined by qRT-PCR technique. Results The results of this study indicated that Prunus armeniaca significantly inhibited proliferation of cells in concentration-dependent manner in all the time of incubation (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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