| Autor: |
Leroux Denis, Alonso Florian, Coent Tanguy, Garros Maeva, Montvernay Régis, Le Bihan Yann, Fulchiron Corine |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
|
| Zdroj: |
EPJ Web of Conferences, Vol 287, p 03017 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
2100-014X |
| DOI: |
10.1051/epjconf/202328703017 |
| Popis: |
To create an affordable clinical test for use in more decentralized settings, we are developing a multispectral imaging system based on a filter wheel and a smartphone. Our application is the label-free identification of uropathogens from images of bacterial colonies directly on a non-chromogenic culture medium. Using feature selection techniques, we have previously shown through calculations, the possibility to move from hyper- to multi- spectral imaging by exploiting less than 8 spectral bands. Here, we confirm our findings by testing a database of true multispectral images acquired using a filter wheel holding up to 22 dichroic bandpass filters with a 10 nm bandwidth. Performance is reported for 5 species using only 6 filters. Probabilistic SVM algorithms were implemented to allow to reject species other than the targeted most prevalent uropathogens as it is crucial to keep the false-positive rate low. Evolution of specificity and sensitivity with probability threshold are discussed in the light of probability frequency distributions. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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