Effect of 7-dehydrocholesterol and cholesterol-loaded cyclodextrins on bull sperm motility during short term storage

Autor: İNANÇ ME, TEKİN K, OLĞAÇ KT, ÖZEN D, STELLETTA C, UYSAL O, DAŞKIN A
Jazyk: angličtina
Rok vydání: 2017
Předmět:
Zdroj: Kafkas Universitesi Veteriner Fakültesi Dergisi, Vol 23, Iss 4, Pp 661-664 (2017)
Druh dokumentu: article
ISSN: 1309-2251
75618826
DOI: 10.9775/kvfd.2017.17181
Popis: The study was conducted to determine the effect of 7-dehydrocholesterol loaded cyclodextrin (7-DHCLC) and cholesterol-loaded cyclodextrin (CLC) on changes of bull sperm motility during short term storage (+4°C). Collected ejaculates were pooled and divided into 7 groups, as following one control (C); three 7-DHCLC and the other three CLC concentrations. Diluted semen samples were transferred and stored at +4°C and sperm motility was analysed in determined intervals during three days storage period with computer aided sperm analyse (CASA) system. Motility decreased in all groups; however, 7-DHCLC and CLC groups motility decreased in the next two days gradually. But, CLC 1.5 mg/120x106 group were identified as the best groups for protecting motility in short time preservation. In conclusion, adding different rates of 7-DHCLC and CLC to semen extender preserved motility for three days in bull semen stored at +4°C.
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