DOMESTIC BUTANOL-PRODUCING STRAINS OF THE Clostridium GENUS

Autor: O. O. Tigunova, N. Ye. Beiko, H. S. Andriiash, S. H. Pryiomov, S. M. Shulga
Jazyk: English<br />Ukrainian
Rok vydání: 2017
Předmět:
Zdroj: Biotechnologia Acta, Vol 10, Pp 34-42 (2017)
Druh dokumentu: article
ISSN: 2410-7751
2410-776X
DOI: 10.15407/biotech10.01.034
Popis: The aim of the work was to summarize the results of own research concerning obtaining butanol producing strains of Clostridium genus, to identify them by physiological, morphological and genetic methods. Further study of characteristics and biological features of the strains, and various approaches in biotechnological process of butanol production are discussed. The work includes methods to increase butanol accumulation by producer strains. Perspectives of using chemical mutagenesis in Clostridia as a method of increasing butanol production are considered. The feasibility of using non-food raw material as a substrate for fermentation is discussed. Different methods of pretreatment and their impact on the accumulation of butanol in the liquid medium are compared. Butanol accumulation is shown to increase significantly if the synthesis precursors are added as components of enzymatic medium, and the “reverse bard” is used to reduce waste production without affecting the level of butanol synthesis. The problem of conservation of producing strains is given, and protective medium for microorganisms during the freeze-drying is defined.
Databáze: Directory of Open Access Journals