Molecular Identification of Turnip Mosaic virus (TuMV) in Hoary Mustard (Herschfeldia incana) From Iran
| Autor: | M. A. Sabokkhiz, B. Jafarpour, F. Shahriari Ahmadi, S. Tarighi, M.R. Safarnejad |
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| Jazyk: | perština |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Majallah-i ḥifāẓat-i giyāhān, Vol 29, Iss 2, Pp 231-237 (2016) |
| Druh dokumentu: | article |
| ISSN: | 2008-4749 2423-3994 |
| DOI: | 10.22067/jpp.v29i2.31558 |
| Popis: | Turnip mosaic virus (TuMV) is amember of the Potyvirus genus within the Potyviridae family and is one the most important viruses infecting Brassicaceae plants. In April 2012, suspicious symptoms of a viral disease such as mosaic, stunting and malformation were observed on Herschfeldia incana. The collected samples were tested using reverse transcription-polymerase chain reaction (RT-PCR) with specific primers corresponding to TuMV coat protein gene. Amplified fragment (986bp) was first purified and then directly sequenced. Analysis of its CP nucleotide and amino acid sequence revealed 85.42-89.58 % and 91.64-95.12% similarity to those of 31 TuMV isolates from other countries respectively. Phylogenetic tree was constructed by MEGA6 software using neighbor joining method. The results showed that the TuMV isolate and 3 Iranian isolates have been clustered into the basal-B group. |
| Databáze: | Directory of Open Access Journals |
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