| Autor: |
Wayan Wariata, Made Sriasih, Anwar Rosyidi, Muhamad Ali, Sulaiman Ngongu Depamede |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Journal of Advanced Veterinary and Animal Research, Vol 9, Iss 1, Pp 122-127 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2311-7710 |
| DOI: |
10.5455/javar.2022.i576 |
| Popis: |
Objective: The main objective of this study is to design a synthetic vaccine from the binder of sperm-1 (BSP1). Materials and Methods: This study was carried out using bioinformatics-related techniques. BSP-1 has been chosen as one of the biomarkers of a ruminant's male fertility. We hypothesize that the BSP1 synthetic vaccines, which contain T-cell epitopes, can produce antibodies more effectively for the development of a sperm fertility detection kit. A sequence of BSP-1 peptides A0A0K1YXR5 from Bubalus bubalis (Domestic water buffalo) origin has been decided to be used to develop the peptide vaccine. Results: In this study, we succeeded in making synthetic vaccines from BSP-1 with a peptide sequence of LPEDSVPDEERVFPFTYRNRKHF. The three-dimensional theoretical prediction analy¬sis of the peptide binding pattern to its ligand, as well as the molecular docking, has also been revealed. Conclusions: A synthetic vaccine from the BSP-1 has been developed in this study with the amino acid sequence LPEDSVPDEERVFPFTYRNRKHF, which is buffer-soluble, and the three-dimensional theoretical prediction analysis of the peptide binding pattern of BSP-1 to its ligand, as well as molecular docking, has also been revealed. [J Adv Vet Anim Res 2022; 9(1.000): 122-127] |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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