The effects of calcium silicate cement/fibroblast growth factor-2 composite on osteogenesis accelerator in human dental pulp cells
| Autor: | Buor-Chang Wu, Su-Chung Youn, Chia-Tze Kao, Shu-Ching Huang, Chi-Jr Hung, Ming-Yung Chou, Tsui-Hsien Huang, Ming-You Shie |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Journal of Dental Sciences, Vol 10, Iss 2, Pp 145-153 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1991-7902 14770059 |
| DOI: | 10.1016/j.jds.2013.12.003 |
| Popis: | Background/purpose: To examine the effects of fibroblast growth factor-2 (FGF-2)/calcium silicate (CS) cement on material characters and in vitro primary human dental pulp cell (hDPC) behavior. Materials and methods: Setting time and diametral tensile strength (DTS) of CS and CS/FGF-2 composite were measured. PrestoBlue assay was used for evaluating primary hDPC proliferation. Alkaline phosphatase and osteocalcin expression in HDPCs cultured on the specimens were determined by enzyme-linked immunosorbent assay. One-way analysis of variance was used to evaluate the significance of the differences between the mean values. Results: Setting time and DTS of CS were not significantly different (P > 0.05) between CS hydration with H2O or FGF-2. Cell proliferation and osteogenic properties increased significantly (P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|