A silicic acid adsorption method for the determination of ganglioside sialic acid

Autor: R.H. Mccluer, E.H. Coram, H.S. Lee
Jazyk: angličtina
Rok vydání: 1962
Předmět:
Zdroj: Journal of Lipid Research, Vol 3, Iss 2, Pp 269-274 (1962)
Druh dokumentu: article
ISSN: 0022-2275
DOI: 10.1016/S0022-2275(20)40440-7
Popis: A semimicro method for the analysis of ganglioside sialic acid is presented. The method is based upon the fact that brain gangliosides can be adsorbed on a small amount of silicic acid from 92% n-butanol and eluted with methanol. Recovery of added gangliosides was found to be 93.5%, and the coefficient of variation for the analysis of 5 to 50 mg of tissue was found to be ±4.4%.
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