Green Fluorescent Protein Labeling of Cytoskeletal Structures—Novel Targeting Approach Based on Leucine Zippers
| Autor: | B.-Z. Katz, D. Krylov, S.-i. Aota, M. Olive, C. Vinson, K.M. Yamada |
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| Jazyk: | angličtina |
| Rok vydání: | 1998 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 25, Iss 2, Pp 298-304 (1998) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/98252rr01 |
| Popis: | Green fluorescent protein (GFP) is a valuable marker for intracellular protein localization. However, the fusion of GFP with structural proteins can alter their properties, resulting in a loss of fusion protein localization, decreased GFP fluorescence or both. We describe a novel targeting approach based on noncovalent heterodimerization of GFP and cytoplasmic structural proteins. The formation of structural protein/GFP complexes was mediated by modified leucine zipper protein spacers designed to form high-affinity heterodimers. The complexes localized accurately to specific sites within cells, providing selective fluorescence labeling of subcellular structures such as microfilaments or focal contacts. |
| Databáze: | Directory of Open Access Journals |
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