| Autor: |
Avinash Waghray, Néstor Saiz, Anitha D. Jayaprakash, Ana G. Freire, Dmitri Papatsenko, Carlos-Filipe Pereira, Dung-Fang Lee, Ran Brosh, Betty Chang, Henia Darr, Julian Gingold, Kevin Kelley, Christoph Schaniel, Anna-Katerina Hadjantonakis, Ihor R. Lemischka |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Stem Cell Reports, Vol 5, Iss 1, Pp 97-110 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
2213-6711 |
| DOI: |
10.1016/j.stemcr.2015.05.009 |
| Popis: |
Tbx3, a member of the T-box family, plays important roles in development, stem cells, nuclear reprogramming, and cancer. Loss of Tbx3 induces differentiation in mouse embryonic stem cells (mESCs). However, we show that mESCs exist in an alternate stable pluripotent state in the absence of Tbx3. In-depth transcriptome analysis of this mESC state reveals Dppa3 as a direct downstream target of Tbx3. Also, Tbx3 facilitates the cell fate transition from pluripotent cells to mesoderm progenitors by directly repressing Wnt pathway members required for differentiation. Wnt signaling regulates differentiation of mESCs into mesoderm progenitors and helps to maintain a naive pluripotent state. We show that Tbx3, a downstream target of Wnt signaling, fine tunes these divergent roles of Wnt signaling in mESCs. In conclusion, we identify a signaling-TF axis that controls the exit of mESCs from a self-renewing pluripotent state toward mesoderm differentiation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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