| Autor: |
Trogl J., Ripp S., Kuncova G., Sayler G.S., Demnerova K. |
| Jazyk: |
English<br />Serbian |
| Rok vydání: |
2003 |
| Předmět: |
|
| Zdroj: |
Hemijska Industrija, Vol 57, Iss 12, Pp 596-599 (2003) |
| Druh dokumentu: |
article |
| ISSN: |
0367-598X |
| DOI: |
10.2298/HEMIND0312596T |
| Popis: |
The bioluminescent bioreporter Pseudomonas fluorescens HK44, the whole cell bacterial biosensor that responds to naphthalene and its metabolites via the production of visible light, was immobilized into a silica matrix by the sol-gel technique. The bioluminescence intensities were measured in the maximum of the bioluminescence band at X = 500 nm. The immobilized cells (>105 cells per g silica matrix) produced light after induction by salicylate (cone. > 10 g/l), naphthalene and aminobenzoic acid. The bioluminescence intensities induced by 2,3-dihydroxynaphthalene 3-hydroxybenzoic acid and 4-hydroxybenzoic acid were comparable to a negative control. The cells in the silica layers on glass slides produced light in response to the presence of an inductor at least 8 months after immobilization, and >50 induction cycles. The results showed that these test slides could be used as assays for the multiple determination of water pollution. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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