Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) of urinary protein in acute kidney injury

Autor: Sufi M Suhail, K T Woo, H K Tan, K S Wong
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Saudi Journal of Kidney Diseases and Transplantation, Vol 22, Iss 4, Pp 739-745 (2011)
Druh dokumentu: article
ISSN: 1319-2442
Popis: Recent experimental and clinical studies have shown the importance of urinary proteomics in acute kidney injury (AKI). We analyzed the protein in urine of patients with clinical AKI using sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) for its diagnostic value, and followed them up for 40 months to evaluate prognosis. Urine from 31 consecutive cases of AKI was analyzed with SDS-PAGE to determine the low, middle and high molecular weight proteins. Fractional excretion of sodium (FENa) was estimated from serum and urine creatinine and sodium (Na). The cases were followed-up for 40 months from the end of the recruitment of study cases. Glomerular protein was higher in the hematuria group when compared with the non-hematuria group (P
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