Quantificação sérica de vitamina C por CLAE-UV e estudo de estabilidade
| Autor: | Marília Baierle, André de Bairros, Ana Paula Moreira, Rachel Bulcão, Miguel Roehrs, Fernando de Freitas, Juliano Durgante, Natália Brucker, Mariele Charão, Solange Cristina Garcia |
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| Jazyk: | English<br />Spanish; Castilian<br />Portuguese |
| Rok vydání: | 2012 |
| Předmět: | |
| Zdroj: | Química Nova, Vol 35, Iss 2, Pp 403-407 (2012) |
| Druh dokumentu: | article |
| ISSN: | 1678-7064 0100-4042 |
| DOI: | 10.1590/S0100-40422012000200030 |
| Popis: | Vitamin C, an exogenous antioxidant, is essential to human health. In this study, a method was validated to serum vitamin C quantification by HPLC-UV. Its stability with and without the use of tris [2-carboxy-ethyl] phosphine hydrochloride (TCEP), at -20 and -80 °C, in serum and supernatant were also evaluated. Analysis showed r² > 0.99, precision CV% < 15% and % bias < 15%, being linear, precise and accurate. The stability test revealed that using TCEP in serum storage at -20 and -80 °C or in supernatant at -80 °C the vitamin C levels remain stable for 30 and 12 days, respectively. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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