Data on preparation and characterization of an insect odorant receptor based biosensor

Autor: Roshan Khadka, Nihan Aydemir, Colm Carraher, Cyril Hamiaux, Damon Colbert, Jamal Cheema, Jenny Malmström, Andrew Kralicek, Jadranka Travas-Sejdic
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Data in Brief, Vol 21, Iss , Pp 2142-2148 (2018)
Druh dokumentu: article
ISSN: 2352-3409
DOI: 10.1016/j.dib.2018.11.018
Popis: Insect Odorant receptors (OrXs) can be used as the recognition element in a biosensor as they demonstrate high levels of sensitivity and selectivity towards volatile organic compounds. Herein, we describe a method to express and purify insect odorant receptors and reconstitute them into artificial lipid bilayers (liposomes). These OrX/liposomes were covalently attached to a gold surface and characterized using quartz crystal microbalance with dissipation monitoring (QCM-D). The interaction of OrX/liposomes immobilized on a gold surface to positive and negative odorants were studied by means of electrochemical impedance spectroscopy (EIS) and QCM-D. The data presented in this article are related to the research article titled “An ultrasensitive electrochemical impedance-based biosensor using insect odorant receptors to detect odorants” [1].
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