| Autor: |
Christopher J. Bergin, Yannick D. Benoit |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
STAR Protocols, Vol 3, Iss 1, Pp 101218- (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2022.101218 |
| Popis: |
Summary: Organoids can enable the study of solid tumors initiated from a single cancer stem cell (CSC) ex vivo. We describe a serial tumor organoid plating protocol using primary colorectal cancer (CRC) tissues as a rapid and cost-efficient approach to evaluate the impact of therapeutic interventions on CSC functions. We detail the isolation of primary colorectal CSCs, organoid embedding, serial passaging, and CSC-related analytical techniques.For complete details on the use and execution of this protocol, please refer to Masibag et al. (2021) and Bergin et al. (2021). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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