Alpha-defensins determination in different types of synovial fluid and parallel collected serum samples by ELISA
| Autor: | Pavlina Kusnierova, Iveta Bystronova, Pavel Walder, Rudolf Hlubek, Frantisek Vsiansky, David Stejskal |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | Biomedical Papers, Vol 165, Iss 4, Pp 367-374 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1213-8118 1804-7521 |
| Popis: | Aims. The study aims were to verify the serum (S) and synovial fluid (SF) reference intervals (RIs) for human neutrophil defensins (HNP1-3); measure S and SF defensin concentrations in different types of SF, including non-inflammatory, inflammatory non-pyogenic, inflammatory pyogenic, and hemorrhagic; and to compare the HNP1-3 concentrations in SF and S with those of other inflammatory biomarkers. Methods. SF and S samples were collected from 92 patients. HNP1-3 concentrations were determined using enzyme-linked immunosorbent assays; glucose, lactate, interleukin-6, and procalcitonin using an automatic analyzer; and presepsin using a Pathfast system. There were 61 non-inflammatory, 11 inflammatory non-pyogenic, 11 inflammatory pyogenic, and 9 hemorrhagic SF. Non-inflammatory SF was divided into non-inflammatory normal and non-inflammatory osteoarthritis. The former was used to estimate the HNP1-3 RI in SF and S. Results. The estimated HNP1-3 RIs of SF and S were 12.47-437.42 mg/L and 5.45-44.75 µg/L, respectively. HNP1-3 differed significantly between S and SF and individual groups of SF (P |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
|