| Autor: |
Hathaigan Kokkaew, Supawan Thawornchinsombut, Jae Won Park |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Songklanakarin Journal of Science and Technology (SJST), Vol 38, Iss 4, Pp 439-447 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
0125-3395 |
| Popis: |
Response surface methodology (RSM) was performed to maximize the mercury (Hg) reduction from yellowfin tuna (Thunnus albacare) by products protein isolates (YBPI). The optimal condition of Hg reduction (89.3%) was 10.5 mM CaCl 2 and a Water:YB of 12.9:1, while other variables were fixed at 5 mM citric acid, 60 min incubation, pH 11 and 8,000 x g for 15 min of centrifugation. At these conditions, the significant protein recovery (80.1%) was obtained. Hydrolysates sequentially hydrolyzed with G6 followed by GN exhibited the highest angiotensin I-converting enzyme (ACE) inhibitory activity than other enzyme preparations. Fractionated yellowfin tuna by products protein hydrolysate (YBPH) increased in ACE-inhibitory activity when peptide size decreased. In-vitro gastrointestinal (GI) digestion significantly increased bioactive property. ACEinhibitory activity of YBPH with and without simulated GI digestion significantly increased after incubating against ACE, demonstrating pro-drug type peptides. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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