| Autor: |
Stephen Magambo, Alice Nabatanzi, Titus Alicai, Enoch Wembabazi, Ketra Oketcho, Immaculate Nakalembe, Henry Wagaba |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Scientific African, Vol 23, Iss , Pp e02039- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2468-2276 |
| DOI: |
10.1016/j.sciaf.2023.e02039 |
| Popis: |
Friable embryogenic callus (FEC) production is a prerequisite for the genetic transformation of cassava (Manihot esculenta Crantz). This process necessitates the development of organized embryogenic structures (OES) as a precursor. However, the production of OES and FECs in cassava is dependent on the genotype. This study aimed to optimize somatic embryo production in elite Ugandan cassava genotypes (NASE13, NASE19, and NAROCASS1) and demonstrate genetic transformation using the enhanced green fluorescent protein (eGFP) gene. OES were induced by initiating leaf-lobe explants on Murashige and Skoog (MS) media at various concentrations of 24D and picloram followed by friable embryogenic callus (FEC) production and regeneration. There were highly significant differences among the different media compositions in the percentage response to OES formation (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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