The Effect of N-Acetyl-Cysteine on NRF 2 Antioxidant Gene Expression in Asthenoteratozoospermia Men: A Clinical Trial Study
| Autor: | Rahil Jannatifar, Kazem Parivar, Nasim Hayati Roodbari, Mohammad Hossein Nasr-Esfahani |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: | |
| Zdroj: | International Journal of Fertility and Sterility, Vol 14, Iss 3, Pp 171-175 (2020) |
| Druh dokumentu: | article |
| ISSN: | 2008-076X 2008-0778 |
| DOI: | 10.22074/ijfs.2020.44411 |
| Popis: | Background: One of the important factor associated with male infertility is high production of reactive oxygen species(ROS). The main function of Nuclear factor erythroid 2-related factor 2 (NRF 2) is to activate the cellular antioxidantresponse by inducing the transcription of a wide array of genes that can combat the harmful effects of factorssuch as oxidative stress. The purpose of this study was to evaluate the effect of N-acetyl-L-cysteine (NAC), as anantioxidant drug, on NRF 2 Gene Expression in Asthenoteratozoospermia Men.Materials and Methods: In this randomized, blinded clinical trial study, included 50 infertile men with asthenoteratozoospermia,who received NAC (600 mg, three times daily). Sperm parameters analyzed according to the world health organization(WHO; 2010). Sperm DNA fragmentation, relative NRF 2 expression, and seminal plasma level of antioxidant enzymeswere measured by TUNEL assay, reverse transcription polymerase chain reaction (RT-PCR) and ELISA test, respectively.Results: After NAC treatment, findings showed a significant increase in sperm concentration and motility comparedto pre-treatment status, whereas the percentage of abnormal morphology and DNA fragmentation was significantlydecreased (p |
| Databáze: | Directory of Open Access Journals |
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