Facilitating Imaging Mass Spectrometry of Microbial Specialized Metabolites with METASPACE

Autor: Don D. Nguyen, Veronika Saharuka, Vitaly Kovalev, Lachlan Stuart, Massimo Del Prete, Kinga Lubowiecka, René De Mot, Vittorio Venturi, Theodore Alexandrov
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Metabolites, Vol 11, Iss 8, p 477 (2021)
Druh dokumentu: article
ISSN: 11080477
2218-1989
DOI: 10.3390/metabo11080477
Popis: Metabolite annotation from imaging mass spectrometry (imaging MS) data is a difficult undertaking that is extremely resource intensive. Here, we adapted METASPACE, cloud software for imaging MS metabolite annotation and data interpretation, to quickly annotate microbial specialized metabolites from high-resolution and high-mass accuracy imaging MS data. Compared with manual ion image and MS1 annotation, METASPACE is faster and, with the appropriate database, more accurate. We applied it to data from microbial colonies grown on agar containing 10 diverse bacterial species and showed that METASPACE was able to annotate 53 ions corresponding to 32 different microbial metabolites. This demonstrates METASPACE to be a useful tool to annotate the chemistry and metabolic exchange factors found in microbial interactions, thereby elucidating the functions of these molecules.
Databáze: Directory of Open Access Journals
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