| Autor: |
Choumessi Aphrodite T, Danel Mathieu, Chassaing Stefan, Truchet Isabelle, Penlap Véronique B, Pieme Anatole, Asonganyi Tazoacha, Ducommun Bernard, Valette Annie |
| Jazyk: |
angličtina |
| Rok vydání: |
2012 |
| Předmět: |
|
| Zdroj: |
Cell Division, Vol 7, Iss 1, p 8 (2012) |
| Druh dokumentu: |
article |
| ISSN: |
1747-1028 |
| DOI: |
10.1186/1747-1028-7-8 |
| Popis: |
Abstract Background Xylopia aethiopica, a plant found throughout West Africa, has both nutritional and medicinal uses. The present study aims to characterize the effects of extracts of this plant on cancer cells. Results We report that X. aethiopica extract prepared with 70% ethanol has antiproliferative activity against a panel of cancer cell lines. The IC50 was estimated at 12 μg/ml against HCT116 colon cancer cells, 7.5 μg/ml and > 25 μg/ml against U937 and KG1a leukemia cells, respectively. Upon fractionation of the extract by HPLC, the active fraction induced DNA damage, cell cycle arrest in G1 phase and apoptotic cell death. By using NMR and mass spectrometry, we determined the structure of the active natural product in the HPLC fraction as ent-15-oxokaur-16-en-19-oic acid. Conclusion The main cytotoxic and DNA-damaging compound in ethanolic extracts of Xylopia aethiopica is ent-15-oxokaur-16-en-19-oic acid. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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