A high-fructose diet induces changes in pp185 phosphorylation in muscle and liver of rats
| Autor: | M. Ueno, R.M.N. Bezerra, M.S. Silva, D.Q. Tavares, C.R. Carvalho, M.J.A. Saad |
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| Jazyk: | angličtina |
| Rok vydání: | 2000 |
| Předmět: | |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 33, Iss 12, Pp 1421-1427 (2000) |
| Druh dokumentu: | article |
| ISSN: | 0100-879X 1414-431X |
| DOI: | 10.1590/S0100-879X2000001200004 |
| Popis: | Insulin stimulates the tyrosine kinase activity of its receptor resulting in the tyrosine phosphorylation of pp185, which contains insulin receptor substrates IRS-1 and IRS-2. These early steps in insulin action are essential for the metabolic effects of insulin. Feeding animals a high-fructose diet results in insulin resistance. However, the exact molecular mechanism underlying this effect is unknown. In the present study, we determined the levels and phosphorylation status of the insulin receptor and pp185 (IRS-1/2) in liver and muscle of rats submitted to a high-fructose diet evaluated by immunoblotting with specific antibodies. Feeding fructose (28 days) induced a discrete insulin resistance, as demonstrated by the insulin tolerance test. Plasma glucose and serum insulin and cholesterol levels of the two groups of rats, fructose-fed and control, were similar, whereas plasma triacylglycerol concentration was significantly increased in the rats submitted to the fructose diet (P |
| Databáze: | Directory of Open Access Journals |
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