| Autor: |
Deiviane A. Calegar, Beatriz Coronato-Nunes, Polyanna A.A. Bacelar, Kerla J.L. Monteiro, Jéssica P. Santos, Brenda B.C. Evangelista, Lauren H. Jaeger, Filipe A. Carvalho-Costa |
| Jazyk: |
English<br />Portuguese |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Pesquisa Veterinária Brasileira, Vol 43 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1678-5150 |
| DOI: |
10.1590/1678-5150-pvb-7330 |
| Popis: |
ABSTRACT: This study performed a molecular detection and characterization of Giardia duodenalis infecting pigs, goats and sheep in rural and peri-urban communities in the state of Piauí, northeastern Brazil, and proposed phylogenetic relationships among the characterized parasites. We assessed 52 fecal samples from pigs, 13 from goats, and 10 from sheep. A fragment of the β-giardin locus was PCR-amplified and sequenced. Overall, PCR-based G. duodenalis positivity was 11/52 (21.2%) in pigs, 2/13 (15.4%) in goats, and 2/10 (20%) in sheep. Seven out of 15 successfully amplified samples could be sequenced: three from pigs, two from goats, and two from sheep. Parasites from different hosts were found to belong to sub-assemblage AII. The phylogenetic analyses of the original G. duodenalis AII β-giardin sequences obtained from distinct host species and sequences of G. duodenalis recovered from humans available in GenBank suggest that the parasites are genetically related, supporting a local scenario of cross-host transmission. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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