| Autor: |
H Kadowaki, J E Evans, R H McCluer |
| Jazyk: |
angličtina |
| Rok vydání: |
1984 |
| Předmět: |
|
| Zdroj: |
Journal of Lipid Research, Vol 25, Iss 10, Pp 1132-1139 (1984) |
| Druh dokumentu: |
article |
| ISSN: |
0022-2275 |
| DOI: |
10.1016/S0022-2275(20)37723-3 |
| Popis: |
A method for the separation of molecular species of brain monosialogangliosides by high-performance liquid chromatography is described. GM4, GM3, GM2, and GM1 were purified from human brain and their individual molecular species were separated on a C18 reversed-phase column. Peaks were identified by mass spectrometry of the intact ganglioside, by gas-liquid chromatography of the fatty acids, and by high-performance liquid chromatography of the long chain bases. A characteristic elution sequence of molecular species permitted their identification based upon their retention times on the reversed-phase column. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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