| Autor: |
Mauro Carcheri, Ilaria Chiossone, Paola Milano, Anna Alabiso, Fabrizia Capanna, Annamaria Ferretti, Licia Muselli, Caterina Oliveri, Laura Tassi, Roberto Capuzzo |
| Jazyk: |
English<br />Italian |
| Rok vydání: |
2010 |
| Předmět: |
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| Zdroj: |
Microbiologia Medica, Vol 25, Iss 1 (2010) |
| Druh dokumentu: |
article |
| ISSN: |
2280-6423 |
| DOI: |
10.4081/mm.2010.2477 |
| Popis: |
The aim of this study was to evaluate the results of the determination of IgG and IgM against TORCH complex antigens obtained by using a new automatic random-access (RAD 120 – RADIM/SEAC) instrument based on FEIA method, in comparison with the results obtained for the same analytes by using a traditional analyzer based on a coated microplate ELISA method (ALISEI-RADIM/SEAC).The analysis of the discordances between the results was made with the system ELFA (VIDAS – bioMérieux), taken as a reference test. The general concordance of the results obtained with the FEIA and the ELISA was over 97.00%. For the single analyte as Toxo IgG,Toxo IgM, Rubeo IgM, CMV IgG, CMV IgM and HSV 2 IgG, the concordance was above 96.00% and 100.00% for the Rubeo IgG and HSV 1 IgG. For the HSV 1/2 IgM, the FEIA/ELISA results show lower concordance, but still reaching 90.16%. For the IgG and IgM dosage, the FEIA seems to give more positive results than the ELISA. If the discordances FEIA/ELISA concern the IgG, the ELISA seems to agree better with the ELFA results. For the IgM dosage, all the FEIA positive/ELISA negative results have been confirmed as negative at ELFA. In conclusion the new technology FEIA/RAD120 gave results which can be comparable with those obtained at the traditional ELISA as well at the alternative ELFA. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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