Molecular Species Delimitation Using COI Barcodes of Mealybugs (Hemiptera: Pseudococcidae) from Coffee Plants in Espírito Santo, Brazil

Autor: Pablo Viana Oliveira, Alexandre Rosa dos Santos, Emily Lopes Olive, Karolinni Bianchi Britto, Francine Alves Nogueira de Almeida, Vitor Cezar Pacheco da Silva, Carolina Barros Machado, Maurício José Fornazier, José Aires Ventura, Mark Paul Culik, Greiciane Gaburro Paneto
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Diversity, Vol 15, Iss 2, p 305 (2023)
Druh dokumentu: article
ISSN: 1424-2818
DOI: 10.3390/d15020305
Popis: Mealybugs are insects belonging to the family Pseudococcidae. This family includes many plant-pest species with similar morphologies, which may lead to errors in mealybug identification and delimitation. In the present study, we employed molecular-species-delimitation approaches based on distance (ASAP) and coalescence (GMYC and mPTP) methods to identify mealybugs collected from coffee and other plant hosts in the states of Espírito Santo, Bahia, Minas Gerais, and Pernambuco, Brazil. We obtained 171 new COI sequences, and 565 from the BOLD Systems database, representing 26 candidate species of Pseudococcidae. The MOTUs estimated were not congruent across different methods (ASAP-25; GMYC-30; mPTP-22). Misidentifications were revealed in the sequences from the BOLD Systems database involving Phenacoccus solani × Ph. solenopsis, Ph. tucumanus × Ph. baccharidis, and Planacoccus citri × Pl. minor species. Ten mealybug species were collected from coffee plants in Espírito Santo. Due to the incorrect labeling of the species sequences, the COI barcode library of the dataset from the database needs to be carefully analyzed to avoid the misidentification of species. The systematics and taxonomy of mealybugs may be improved by integrative taxonomy which may facilitate the integrated pest management of these pests.
Databáze: Directory of Open Access Journals
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