Increased sensitivity using real-time dPCR for detection of SARS-CoV-2
| Autor: | Kyra Duong, Jiajia Ou, Zhaoliang Li, Zhaoqing Lv, Hao Dong, Tao Hu, Yunyun Zhang, Ava Hanna, Skyler Gordon, Gogce Crynen, Steven R Head, Phillip Ordoukhanian, Yan Wang |
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| Jazyk: | angličtina |
| Rok vydání: | 2021 |
| Předmět: | |
| Zdroj: | BioTechniques, Vol 70, Iss 1, Pp 7-20 (2021) |
| Druh dokumentu: | article |
| ISSN: | 1940-9818 0736-6205 |
| DOI: | 10.2144/btn-2020-0133 |
| Popis: | A real-time dPCR system was developed to improve the sensitivity, specificity and quantification accuracy of end point dPCR. We compared three technologies – real-time qPCR, end point dPCR and real-time dPCR – in the context of SARS-CoV-2. Some improvement in limit of detection was obtained with end point dPCR compared with real-time qPCR, and the limit of detection was further improved with the newly developed real-time dPCR technology through removal of false-positive signals. Real-time dPCR showed increased linear dynamic range compared with end point dPCR based on quantitation from amplification curves. Real-time dPCR can improve the performance of TaqMan assays beyond real-time qPCR and end point dPCR with better sensitivity and specificity, absolute quantification and a wider linear range of detection. |
| Databáze: | Directory of Open Access Journals |
| Externí odkaz: |
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