High frequency plant regeneration from the mature seeds of Garcinia indica

Autor: M. Baskaran, S. Krishnan
Jazyk: angličtina
Rok vydání: 2011
Předmět:
Zdroj: Biologia Plantarum, Vol 55, Iss 3, Pp 554-558 (2011)
Druh dokumentu: article
ISSN: 1573-8264
DOI: 10.1007/s10535-011-0124-5
Popis: A high frequency plant regeneration system was developed for the production of high yielding elite clones of Garcinia indica via direct organogenesis. A maximum number of 86.2 shoot buds per explant were induced from the mature seed segments cultured on Woody plant medium (WPM) supplemented with 2.0 mg dm-3 N6-benzyladenine and 1 mg dm-3 indole-3-acetic acid. Rooting was achieved on half-strength WPM medium supplemented with 3 mg dm-3 indole-3-butyric acid. Rooted plantlets were acclimatized and transferred to greenhouse for further growth. The highest survival rate of 95 % was recorded using a mixture of garden soil and sand. Histological studies clearly revealed multiple shoot formation from mature seed segments.
Databáze: Directory of Open Access Journals