Effects of intermittent fasting on the histology and mRNA expression of major drug-metabolizing cyp450s in the liver of diabetic mice
| Autor: | Nour Al-Kazimi, Yazun Jarrar, Ghasaq Abdul-Wahab, Ahmad R. Alsayed, Abdalla Madani, Dina Abulebdah, Rami Salem Musleh, Qais Jarrar, Hamzeh J Al-Ameer, Wajdy Al-Awaida, Eman Abdullah |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Libyan Journal of Medicine, Vol 18, Iss 1 (2023) |
| Druh dokumentu: | article |
| ISSN: | 19932820 1819-6357 1993-2820 |
| DOI: | 10.1080/19932820.2023.2270188 |
| Popis: | ABSTRACTIntroduction:There is a variation in drug response among patients who practice intermittent fasting. Alteration in the expression of drug-metabolizing enzymes (DMEs) can affect the pharmacokinetics and drug response.Aims: This research aimed to determine the effect of intermittent fasting on the mRNA expression of major drug-metabolizing cyp450s in the liver of diabetic mice.Methods: Thirty-two male Balb/c mice were divided into four groups; control, nonfasting diabetic, non-diabetic fasting, and diabetic fasting mice. Insulin-dependent diabetes was induced in mice by a single high-dose (250 mg/kg) streptozocin. Mice of non-diabetic and diabetic fasting groups were subjected to 10-day intermittent fasting for 17 hours daily. Then, the mRNA expression of mouse phase I DMEs cyp1a1, cyp2c29, cyp2d9, and cyp3a11 was analyzed using real-time polymerase chain reaction. In addition, the liver of mice in all groups was examined for pathohistological alterations.Results: Diabetes downregulated the mRNA expression of hepatic drug-metabolizing cyp450s in diabetic mice, while intermittent fasting significantly (P |
| Databáze: | Directory of Open Access Journals |
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