Fine-Scale Adaptations to Environmental Variation and Growth Strategies Drive Phyllosphere Methylobacterium Diversity

Autor: Jean-Baptiste Leducq, Émilie Seyer-Lamontagne, Domitille Condrain-Morel, Geneviève Bourret, David Sneddon, James A. Foster, Christopher J. Marx, Jack M. Sullivan, B. Jesse Shapiro, Steven W. Kembel
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: mBio, Vol 13, Iss 1 (2022)
Druh dokumentu: article
ISSN: 2150-7511
DOI: 10.1128/mbio.03175-21
Popis: ABSTRACT Methylobacterium is a prevalent bacterial genus of the phyllosphere. Despite its ubiquity, little is known about the extent to which its diversity reflects neutral processes like migration and drift, versus environmental filtering of life history strategies and adaptations. In two temperate forests, we investigated how phylogenetic diversity within Methylobacterium is structured by biogeography, seasonality, and growth strategies. Using deep, culture-independent barcoded marker gene sequencing coupled with culture-based approaches, we uncovered a considerable diversity of Methylobacterium in the phyllosphere. We cultured different subsets of Methylobacterium lineages depending upon the temperature of isolation and growth (20°C or 30°C), suggesting long-term adaptation to temperature. To a lesser extent than temperature adaptation, Methylobacterium diversity was also structured across large (>100 km; between forests) and small (
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